Composite
Pc-pelB-rD

Part:BBa_K4225007:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-08)


Pc-pelB-rDAO-6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1172
    Illegal BamHI site found at 2100
    Illegal BamHI site found at 2354
    Illegal XhoI site found at 970
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 156
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All parts are taken from biobricks and are codon optimized for our chassis, DH5α.